Recently Niu et al have tried an alternate approach for incorporating UAAs in response to a quadruplet codon

Recently Niu et al have tried an alternate approach for incorporating UAAs in response to a quadruplet codon Utilizing an alternative ribosome, which is also orthogonal was attractive, as it can end up being advanced to specifically acknowledge both modified mRNA using quadruplet codon and also the tRNA aided by the quadruplet anticodon while leaving the variety translational machinery undisturbed. It was shown that the evolved ribosome, Ribo-Q1 can more proficiently translate both triplet and quadruplet codons compared to the indigenous ribosomes . In line with the idea that the tRNA Pyl anticodon circle just isn’t an important determining factor for any acceptance of the cognate PylRS, the authors very first altered the anticodon of UCCU to recognize the codon AGGA. […]